贾第虫的免疫学和致病机理的研究,通常需要获得足量纯化的包囊,或者直接来自感染者粪便,或者由滋养体在体外成囊获得。后者虽然能够较易获得足量而纯净的包囊,但技术性要求和费用都较高。自感染者粪中提取包囊比较经济、实用,但由于粪中混有大量细菌和杂质,不易获得高纯度的蓝氏贾第鞭毛虫包囊。分此,我们经过反复试验,摸索出一种切实可行的提取、纯化包囊的方法,介绍如下: 材料与方法一、病人粪便取自本所门诊病人,年龄4～11岁,多以腹痛或腹泻持续1月至半年为主要症状。粪便直接涂片法检查 Giardia immunology and pathogenic mechanisms of research, usually need to get enough purified cysts, either directly from the infected feces, or from trophozoites obtained in vitro capsule. Although the latter is easier to obtain adequate and pure cysts, the technical requirements and costs are high. It is more economical and practical to extract cysts from fecal infected persons, but because of the large amount of bacteria and impurities mixed in feces, it is not easy to obtain the cysts of high purity Giardia lamblia. In this regard, after repeated experiments, we explored a practical extraction and purification of cysts, described as follows: Materials and methods First, the patient’s stool taken from our clinic patients, aged 4 to 11 years old, and more to abdominal pain or Diarrhea continued from January to six months as the main symptom. Direct smear stool examination