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目的建立HPLC法测定氯化铵甘草口服溶液中吗啡的含量。方法以辛烷基硅烷键合硅胶为填充剂,0.0025mol·L-1庚烷磺酸钠溶液-0.05 mol·L-1磷酸二氢钾溶液-乙腈(42∶42∶16)为流动相,检测波长为220 nm;流速:1.0 ml·min-1;柱温:30℃。结果吗啡在0.001 403~0.014 03 mg·ml-1的范围内线性关系良好(r=1.0000),平均回收率为99.59%,RSD为0.24%(n=9)。结论本方法简便、测定结果准确,重复性好,可用于氯化铵甘草口服溶液中吗啡的含量测定。
Objective To establish an HPLC method for the determination of morphine in oral solution of ammonium chloride licorice. Methods The octylsilane bonded silica gel was used as the filler, and 0.0025 mol·L-1 heptane sulfonate solution-0.05 mol·L-1 potassium dihydrogen phosphate solution-acetonitrile (42:42:16) The detection wavelength was 220 nm; the flow rate was 1.0 ml · min-1; the column temperature was 30 ℃. Results The morphine showed good linearity (r = 1.0000) in the range of 0.001 403-0.014 03 mg · ml-1 with an average recovery of 99.59% and a RSD of 0.24% (n = 9). Conclusion The method is simple, accurate and reproducible. It can be used for the determination of morphine in oral solution of ammonium chloride licorice.