论文部分内容阅读
本文用单克隆抗体OKT_3、OKT_4和OKT_8研究经~(60)C γ线照射损伤的外周血淋巴细胞,包括全T 细胞(OKT_3~+)、辅助性T 细胞(T_(?),OKT_4~+),抑制性T 细胞(T_s,OKT_8~+)及T_H/T_s 比值,经过不同时间培养后的辐射效应。用间接免疫荧光法观察。结果表明:经2.5 Gy 照射的淋巴细胞,培养4和96 h 后,OTK_3~+、OKT_8~+和OKT_8~+的阳性细胞数目,与OGy 对照组相比,均有所减少,同时OKT_4/OKT_8比值均有所增加,说明OKT_8~+细胞的绝对值下降更明显。经2.5 Gy 照射的T 细胞和各亚群的膜抗原,即使体外培养到96小时,亦不能完全恢复正常。
In this study, peripheral blood lymphocytes injuried by ~(60)C γ-rays, including whole T cells (OKT_3~+), helper T cells (T_(?), OKT_4~+ ), The inhibitory T cells (T_s, OKT_8~+) and T_H/T_s ratios were radiated after cultured at different times. Observed with indirect immunofluorescence. The results showed that the number of positive cells of OTK_3~+, OKT_8~+, and OKT_8~+ cells was decreased compared with the OGy control group after 4 and 96 h incubation of lymphocytes irradiated with 2.5 Gy, and OKT_4/OKT_8 The ratios increased, indicating that the absolute value of OKT_8~+ cells decreased more significantly. The 2.5-Gy-irradiated T-cells and the membrane antigens of the various subpopulations did not return to normal even after 96 hours of in vitro culture.