单纯疱诊病毒(HSV)可在业经PHA或其他促有丝分裂素预先刺激过的体外人类白细胞培养物中复制。超微结构研究显示:促有丝分裂素诱导的淋巴母细胞可作为病毒复制的靶细胞。为研究HSV能否在B细胞中复制,作者用Ficoll—Hypaque技术从成人和脐带血标本中分离白细胞;用神经氨睃酶处理过的绵羊红细胞分离T、B淋巴细胞; Simplex virus (HSV) can be replicated in in vitro human leukocyte cultures pre-stimulated with PHA or other mitogen. Ultrastructural studies have shown that mitogen-induced lymphoblasts can be used as target cells for viral replication. To investigate whether HSV can replicate in B cells, the authors isolated leukocytes from adult and umbilical cord blood samples using Ficoll-Hypaque technology; isolated T and B lymphocytes from hemagglutinin-treated sheep erythrocytes;