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Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex.Methods The RP-HPLC method was applied to simultaneously determining six ingredients,geniposidic acid,geniposide,genipin,chlorogenic acid,(+)-pinoresinol-di-β-D-glucopyranoside,and(+)-syringaresinol-di-β-D-glucopyranoside,in the different processed barks of Eucommia ulmoides.Results The valid method with good accuracy could be well used to study the processing technique of E.ulmoides;Besides,target ingredients in E.ulmoide were decreased within 6 h when they were processed.Conclusion Established RP-HPLC is a reliable method which could be used to research the processing technique of the barks of E.ulmoides.Moreover,the result of this study could be provided with significant evidence of processed barks of E.ulmoides.
Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex. Methods The RP-HPLC method was applied to determine six ingredients, geniposidic acid, geniposide, genipin, chlorogenic acid, (+) - pinoresinol-di- β-D-glucopyranoside, and (+) - syringaresinol-di-β-D-glucopyranoside, in the different processed barks of Eucommia ulmoides. Results of the valid method with good accuracy could be well used to study the processing technique of E.ulmoides ; Besides, target ingredients in E.ulmoide were decreased within 6 h when they were processed. Confirmation RP-HPLC is a reliable method which could be used to research the processing technique of the barks of E. ulmoides. Moreover, the result of this study could be provided with significant evidence of processed barks of E.ulmoides.