Histological assessment in peripheral nerve tissue engineering

来源 :Neural Regeneration Research | 被引量 : 0次 | 上传用户:w123youlin
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The histological analysis of peripheral nerve regeneration is one of the most used methods to demonstrate the success of the regeneration through nerve conduits. Nowadays, it is possible to evaluate different parameters of nerve regeneration by using histological, histochemical, immunohistochemical and ultrastructural techniques. The histochemical methods are very sensible and are useful tools to evaluate the extracellular matrix remodeling and the myelin sheath, but they are poorly specific. In contrast, the immunohistochemical methods are highly specific and are frequently used for the identification of the regenerated axons, Schwann cells and proteins associated to nerve regeneration or neural linage. The ultrastructural techniques offer the possibility to perform a high resolution morphological and quantitative analysis of the nerve regeneration. However, the use of a single histological method may not be enough to assess the degree of regeneration, and the combination of different histological techniques could be necessary. The histological analysis of peripheral nerve regeneration is one of the most used methods to demonstrate the success of the regeneration of nerve conduits. Nowadays, it is possible to evaluate different parameters of nerve regeneration by using histological, histochemical, immunohistochemical and ultrastructural techniques. The histochemical Methods are very sensible and are useful tools to evaluate the extracellular matrix remodeling and the myelin sheath, but they are poorly specific. In contrast, the immunohistochemical methods are highly specific and are frequently used for the identification of the regenerated axons, Schwann cells and proteins associated to nerve regeneration or neural linage. The ultrastructural techniques offer the possibility to perform a high resolution morphological and quantitative analysis of the nerve regeneration. However, the use of a single histological method may not be enough to assess the degree of regeneration, and the combination of different histological techniques could be necessary.
其他文献
中国运用活血化瘀这一治则已有悠久历史,活血化瘀法是祖国医学治疗血瘀的总法则。早在《黄帝内经》中已有瘀血病因病机的记载。张仲景的《伤寒论》和《金匮要略》更详述了瘀血
期刊
目的:比较喉罩全麻在悬吊式无气腹腹腔镜手术和CO2气腹腹腔镜手术对循环系统的影响。方法将患者随机分为悬吊组(L组)25例,和有气腹组(T组)25例。两组麻醉诱导后均行机械通气。分别
目的:经股动脉介入髂内动脉化疗在中晚期宫颈癌同步放化疗后局部未控治疗的研究。方法将22例中晚期宫颈癌经同步放化疗后局部未控患者分为静脉化疗组10例、介入化疗组12例,观察
目的:对中老年健康男性骨密度进行统计分析,探讨有效干预措施。方法对400例中老年健康男性进行腰椎骨密度测定,实施健康教育、饮食习惯干预、运动干预等综合干预措施,干预周期12
比较了2010年1月-2012年6月入住我院肿瘤科选择PICC置管的157位患者和2007年1月-2009年12月选择PICC置管的160位患者,认为运用循证护理作为预防处理PICC置管常见并发症是科学
目的:探讨神经内科开展森林脑炎临床护理路径的护理服务受限情况及相关因素。方法采用森林脑炎临床路径流程及巴塞尔护理服务受限量表测量,调查神经内科护理服务受限情况。结果
语音关键词识别和确认方法在语音对话系统中得到了广泛的应用.评价此类系统性能的一个重要指标就是处理非关键词(垃圾)的能力.处理垃圾的传统方法是在离线状态下进行垃圾建模
目的:探讨新疆维吾尔族及当地汉族急性心肌梗死(AMI)的发病危险因素和临床表现及.方法:回顾性对比分析2010年1月~2012年11月于新疆维吾尔自治区人民医院急诊ICU住院的60例维吾
大规模电力网络的故障诊断问题可由分布式人工智能技术有效地解决。文中提出了一种用于分布式故障诊断系统的有效的网络分割方法,能够将大规模电力网络分割为给定数目的连通子
提出了基于最优决策理论的最佳分类器用神经网络实现的方法,并对通信信号的调制类型进行自动识别。这种方法以使类域最大分离为目标,利用神经网络良好的非线性和自适应性,把最优