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目的观察5-羟色胺2A受体阻断剂酮色林对大鼠肝部分切除后肝再生的影响,了解5-羟色胺及其受体在肝脏再生中的作用。方法 80只雄性Wistar大鼠随机分为实验组和对照组。采用肝大部分切除术建立肝再生模型,术后16 h分别给予腹腔内注射酮色林(实验组)和生理盐水(对照组),采用免疫组化及流式细胞技术动态观察并比较两组大鼠术后24、36、48、72 h肝脏Ki67、增殖细胞核抗原的表达情况。结果大鼠肝大部切除术后24、36 h肝脏表达Ki67、增殖细胞核抗原最为活跃,而后表达逐渐下降。实验组大鼠肝脏表达Ki67、增殖细胞核抗原较对照组显著下降(P<0.05)。结论 5-羟色胺2A受体阻断剂酮色林显著抑制大鼠肝大部切除术后的肝脏再生,说明5-羟色胺具有一定的促进肝再生的作用,2A受体是其重要的信号传导受体之一。
Objective To observe the effect of ketanserin, a blocker of serotonin 2A receptor, on hepatic regeneration after partial hepatectomy in rats, and to understand the role of serotonin and its receptors in hepatic regeneration. Methods Eighty male Wistar rats were randomly divided into experimental group and control group. The majority of resection of the liver was used to establish a liver regeneration model. Ketanin (experimental group) and saline (control group) were intraperitoneally injected at 16 hours after operation. Immunohistochemistry and flow cytometry were used to observe and compare the two groups The expression of Ki67 and proliferating cell nuclear antigen in liver of rats at 24, 36, 48, 72 h after operation. Results The hepatic expression of Ki67 was positive at 24 and 36 h after subtotal hepatic resection in rats, and the expression of proliferating cell nuclear antigen was the most active, and then decreased gradually. In the experimental group, the liver expression of Ki67 was significantly lower than that of the control group (P <0.05). Conclusions Ketamine, a 5-HT2A receptor antagonist, significantly inhibits hepatic regeneration after partial hepatectomy in rats, suggesting that serotonin may promote liver regeneration. 2A receptor is an important signaling pathway One of the body.