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6个有代表性的甜莱品种在人工气候室的营养液培养试验表明,甜菜子叶期幼苗对NO_3~-的吸收要经最初2h的适应阶段后才可均匀进行。均匀吸收后,观察到多倍体的吸收量>2倍体,倍性相同品种中E型>NE型>NZ型>Z型的初步结果。不同类型品种间NO_3~-吸收的表观米氏常数(Km,app)、最大吸收速率(Im)有明显差异,除多倍体NZ型品种甜研301的Km,app较低而Im较高外,其余品种的Km,app和Im呈正相关。品种间溢泌液体积、溢泌液中NO_3~-量以及NO_3~-上运速率与品种间Im值的排序一致,NRA的体内测定表明,未经NO_3~-诱导的供试品种子叶的NRA无差异。经NO_3~-诱导的,无论反应液中是否加NO_3~-,子叶NRA在不同类型品种间差异显著,子叶NRA与NO_3~-吸收量、NO_3~-上运速率呈极显著正相关。文中还讨论了上述生理生化指标对考察甜菜种质资源的氮素利用性能和选育氮素利用率高的品种的参考价值。
The experiment of nutrient solution culture in 6 representative sweety varieties in the artificial climate chamber showed that the absorption of NO_3 in the seedlings of the beet cotyledons could be carried out uniformly after the first 2 hours of adaptation. After uniform absorption, the initial results of polyploid uptake> 2-fold, ploidy in the same varieties of E type> NE type> NZ type> Z type were observed. The apparent Michaelis constant (Km, app) and maximum absorption rate (Im) of NO_3 ~ - absorption of different types of cultivars were significantly different. In addition, Km, app and Im were positively correlated with other breeds. The order of NO_3 ~ - amount and NO_3 ~ - uptake rate in intercalary products was the same as that between cultivars. The in vitro NRA assay showed that NRA, which was not induced by NO_3 ~ No difference. NO_3 ~ --induced, no matter what NO_3 ~ - in the reaction solution or not, the cotyledonary NRA had significant difference among different types of cultivars, and the NRA of cotyledons showed extremely significant positive correlation with NO_3 ~ uptake and NO_3 ~ - uptake rate. The paper also discussed the above physiological and biochemical indicators of sugar beet germplasm resources to explore the use of nitrogen and breeding of high nitrogen utilization rate of the reference value.