巨噬细胞的分离纯化是免疫学实验中常用的一项基本技术。分离纯化巨噬细胞的方法甚多包括斑蟊素皮肤发泡法、密度梯度离心法及酶消化收集法等。在这些方法中,有的产量较低,实用价值不大;有的需要昂贵的设备和药品;有的则明显影响巨噬细胞活性。Rabinovitch 提出,在有血 Isolation and purification of macrophages is a commonly used basic technique in immunological experiments. Many methods of isolation and purification of macrophages include cantharidin skin foaming method, density gradient centrifugation and enzyme digestion and collection methods. Some of these methods have low yields and little practical value; some require expensive equipment and drugs; others significantly affect macrophage activity. Rabinovitch suggested that there is blood