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Objective:To evaluate the larvicidal activity of essential oil of Origanum mtijoruna(Lamiaceae)cultivated in Morocco against Culex pipiens(Diptera:Culicidae).Methods:The analysis and the identification of the various constituents of essential oil were carried out by gas chromatography coupled with mass spectrometry.Biological test was performed according to a standard methodology inspired by the World Health Organization protocol with slight modification.Results:This oil mainly consisted of monoterpene and sesquiterpenes.The majority compounds are 4-terpinene(28.96%),y-terpinene(18.57%),α-terpinene(12.72%) and sabinene(8.02%).The lethal concentrations(LC_(50) and LC_(90)) measured for the essential oil Origanum majorana,were respectively of the order of 258.71 mg/L and 580.49 mg/L.Conclusions:The results could be useful in search for newer,safer,and more effective natural larvicidal agents.
Objective: To evaluate the larvicidal activity of essential oil of Origanum mtijoruna (Lamiaceae) cultivated in Morocco against Culex pipiens (Diptera: Culicidae). Methods: The analysis and the identification of the various constituents of essential oils were carried out by gas chromatography coupled with mass spectrometry.Biological test was performed according to a standard methodology inspired by the World Health Organization protocol with slight modification. Results: This oil mainly consisted of monoterpene and sesquiterpenes. the majority compounds are 4-terpinene (28.96%), y-terpinene 18.57%), α-terpinene (12.72%) and sabinene (8.02%). The lethal concentrations (LC 50 and LC 90) were measured for the essential oil Origanum majorana, respectively by the order of 258.71 mg / L and 580.49 mg / L.Conclusions: The results could be useful in search for newer, safer, and more effective natural larvicidal agents.