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[目的]研究X线照射对鼻咽癌细胞株CNE1、CNE2中ATM mRNA表达水平的影响。[方法]采用逆转录酶聚合酶链式反应(RT-PCR)技术,以β-actin为内参照,测定10GyX线照射前后CNE1、CNE2中ATM mRNA表达水平的变化。[结果]在X线照射前,CNE1中ATM mRNA基础水平与CNE2相似;在X线照射后,CNE1中ATM mRNA水平在照射4h后明显升高,12h后恢复;CNE2中ATM mRNA水平在照射后12h内无显著变化。[结论]在具有不同放射敏感性的鼻咽癌细胞株CNE1、CNE2中,ATM mRNA表达的基础水平相似,在经X射线处理后,CNE1和CNE2中ATM mRNA水平变化规律不同。这种ATM mRNA表达差异可能是导致两者放射敏感性不同的原因之一。
[Objective] To study the effect of X-ray irradiation on the expression of ATM mRNA in nasopharyngeal carcinoma cell lines CNE1 and CNE2. [Methods] The expression of ATM mRNA in CNE1 and CNE2 before and after irradiation with 10GyX line was determined by reverse transcriptase polymerase chain reaction (RT-PCR) and β-actin as internal reference. [Result] Before X-ray irradiation, the basic level of ATM mRNA in CNE1 was similar to that of CNE2. After X-ray irradiation, the level of ATM mRNA in CNE1 was significantly increased 4h after irradiation and resumed after 12h. The level of ATM mRNA in CNE2 No significant change within 12h. [Conclusion] The basic level of ATM mRNA expression in nasopharyngeal carcinoma cell lines CNE1 and CNE2 with different radiosensitivity is similar. The changes of ATM mRNA levels in CNE1 and CNE2 after X-ray treatment are different. This difference in ATM mRNA expression may be one of the reasons leading to the difference in radiosensitivity between the two.