AIM:To investigate the effects of vitamin E succinate(VES)on the expression of c-jun gene and protein in human gastriccancer SGC-7901 cells.METHODS:After SGC-7901 cells were treated with VES atdifferent doses(5,10,20 mg·L~(-1))at different time,reversetranscription-PCR technique was used to detect the level ofc-jun mRNA;Western Blot was applied to measure theexpression of c-jun protein.RESULTS:After the cells were treated with VES at 20 mg·L~(-1)for 3 h,the expression rapidly reached its maximum thatwas 3.5 times of UT control(P<0.01).The level of c-junmRNA was also increased following treatment of VES for 6 h.However,the expression after treatment of VES at 5 mg·L~(-1)for 24 h was 1.6 times compared with UT control(P<0.01).Western blot analysis showed that the level of c-jun proteinwas obviously elevated in VES-treated SGC-7901 cells at 20mg·L~(-1)for 3 h.The expression of c-jun protein was graduallyincreased after treatment of VES at 20 mg·L~(-1)for 3,6,12 and24 h,respectively,with an evident time-effect relationship.CONCLUSION:The levels of c-jun mRNA and protein inVES-treated SGC-7901 cells were increased in a dose-andtime-dependent manner;the expression of c-jun wasprolonged by VES,indicating that c-jun is involved in VES-induced apoptosis in SGC-7901 cells. AIM: To investigate the effects of vitamin E succinate(VES) on the expression of c-jun gene and protein in human gastriccancer SGC-7901 cells.METHODS:After SGC-7901 cells were treated with VES atdifferent doses(5,10,20 Mg·L~(-1)) at different time, reverse transcription-PCR technique was used to detect the level of c-jun mRNA; Western Blot was applied to measure the expression of c-jun protein.RESULTS:After the cells were treated with VES At 20 mg·L~(-1) for 3 h, the expression rapidly reached its maximum that was 3.5 times of UT control (P<0.01). The level of c-jun mRNA was also increased following treatment of VES for 6 h. The expression after treatment of VES at 5 mg·L~(-1) for 24 h was 1.6 times compared with UT control (P<0.01). Western blot analysis showed that the level of c-jun proteinwas elevated elevated in VES- SGC-7901 cells at 20mg·L -1 for 3 h.The expression of c-jun protein was graduallyincreased after treatment of VES at 20 mg·L -1 for 3,6,12 and24 h, Respectively,w Ith an evident time-effect relationship.CONCLUSION:The levels of c-jun mRNA and protein inVES-treated SGC-7901 cells were increased in a dose-andtime-dependent manner;the expression of c-jun wasprolonged by VES,indicating that c -jun is involved in VES-induced apoptosis in SGC-7901 cells.