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本文对来源于柯兴氏病病人2例单纯垂体人CTH瘤用单层细胞培养的方法,以N-POMC作为ACTH分泌的指标,详细研亢了癌细胞ACTH分泌的调节。结果丧明cRH和AVP均能兴奋N-POMC分泌,并有协同作用;皮质醇对ACTH瘸细胞N-POMC分泌不抑制反使其分泌反常增加,表明皮质醇对AcTH分泌的负反馈调节消失;β-内啡肽对ACTH瘤细胞有直接兴奋N-POMC允泌的作用,这种作用可被纳洛酮阻断;GHRH_(1-44)、SRIF、AT_Ⅱ和VIP对N-POMC分泌无明显影响。
In this paper, from Cushing’s disease in patients with two cases of simple pituitary CTH tumor monolayer cell culture method to N-POMC as an indicator of ACTH secretion, in detail hyperthyroidism regulate the secretion of ACTH in cancer cells. Results Both cRH and AVP were able to excite N-POMC secretion and had a synergistic effect. Cortisol had no inhibitory effect on the secretion of N-POMC in ACTH lame cells, which caused an abnormal increase of secretion, suggesting that the negative feedback regulation of cortisol on AcTH secretion disappeared. β-endorphin on ACTH tumor cells have a direct excitement of N-POMC secretion role, which can be blocked by naloxone; GHRH_ (1-44), SRIF, AT_ Ⅱ and VIP N-POMC secretion was not significant influences.