目的:选择性去除骨髓移植物中异基因反应性淋巴细胞,诱导异基因特异性低反应性。方法:用携带有FasL基因的重组腺病毒转染BALB/c小鼠来源的DC,并与C57BL/6小鼠脾淋巴细胞共培养,通过混合淋巴细胞培养等方法检测异基因特异性低反应性。结果:转染FasL基因的DC诱导了对BALB/c异基因反应性T淋巴细胞凋亡,2次混合淋巴细胞反应显著降低,但并没有抑制针对第三方的反应。结论:转染FasL基因的DC体外可有效去除骨髓移植物中异基因反应性T淋巴细胞,移植用这种方法处理过的骨髓,有希望在抑制GVHD的同时,不影响移植物抗肿瘤复发和抗感染的功能。 OBJECTIVE: To selectively remove alloreactive lymphocytes in bone marrow transplantation and induce allogeneic specific hyporesponsiveness. METHODS: BALB / c mouse-derived DCs were transfected with the recombinant adenovirus carrying FasL gene and co-cultured with C57BL / 6 mouse splenic lymphocytes. Mixed lymphocyte culture was used to detect allogeneic-specific hyporesponsiveness . Results: DCs transfected with FasL gene induced apoptosis of BALB / c allogeneic T lymphocytes, and the second mixed lymphocyte reaction was significantly reduced, but did not inhibit the response to third parties. CONCLUSION: DC transfected with FasL gene can effectively remove allogeneic reactive T lymphocytes in bone marrow transplantation and transplant the bone marrow treated by this method. It is hoped that GVHD can be inhibited without affecting the anti-tumor recurrence and Anti-infection function.