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NOL(NYC1-like)基因编码的蛋白在体外试验中显示出Chlb还原酶活性,但是该基因序列变异是如何影响水稻持绿表型的目前还没有报道。本研究利用本实验室的533份已测序的核心种质材料,对该基因的序列进行了多样性分析,本研究将出现频率>5%的SNP用于数据分析。该基因含有7种单倍型,Hap1和Hap4中SNP位点大致相同,Hap2、Hap3、Hap6含有相似的SNP位点。在单倍型效应分析中,考虑了核心种质的群体结构影响因素,将每个亚群的单倍型叶绿素含量进行了t-test。结果发现Ind亚群中hap4与hap3的差异极显著,hap3与hap4不仅在第一个外显子(181 bp)处产生非同义突变,而且还含有大量的启动子区的变异位点,这些位点有待于后期的功能验证。通过本实验室的表达谱数据库显示,在用植物激素处理的3份水稻材料幼苗中的NOL基因表达量非常高,这说明NOL基因与植物激素的调控有关,其次发现在衰老的叶片中表达量比较高,说明在叶片衰老后期NOL基因参与叶绿素的降解。研究结果从RNA水平为NOL基因调控叶绿素降解作用机理奠定了理论基础。
The protein encoded by the NOL (NYC1-like) gene shows Chlb reductase activity in vitro, but how the sequence variation of this gene affects the green phenotype of rice has not been reported so far. In our study, 533 sequenced core collection materials were used to analyze the diversity of this gene sequence. In this study, SNPs with> 5% frequency were used for data analysis. The gene contains seven haplotypes, Hap1 and Hap4 SNP sites are roughly the same, Hap2, Hap3, Hap6 contains a similar SNP sites. In the haplotype analysis, the population structure of the core germplasm was considered, and the haplotype chlorophyll content of each subpopulation was t-tested. As a result, it was found that there was a significant difference between hap4 and hap3 in the Ind subpopulation. Hap3 and hap4 not only produced non-synonymous mutations in the first exon (181 bp), but also contained a large number of variation sites in the promoter region The site to be functional validation later. The expression profiling database of our laboratory showed that NOL gene expression was very high in three seedlings of rice plants treated with plant hormones, indicating that the NOL gene is involved in the regulation of plant hormones and secondly in the expression of senescent leaves Is higher, indicating that the NOL gene is involved in the degradation of chlorophyll during late leaf senescence. The results laid the theoretical foundation for the mechanism of chlorophyll degradation regulated by NOL gene at RNA level.