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目的 探讨大蹼铃蟾蛋白提取物对膀胱癌细胞株的抑制作用及对端粒酶的影响。方法 采用肿瘤细胞培养方法 ,检测大蹼铃蟾蛋白提取物对膀胱癌细胞株T2 4、BIU 87、ScaBer的抑制作用 ;以TRAP PCR ELISA方法检测大蹼铃蟾蛋白提取物对膀胱癌细胞株端粒酶表达的影响。结果 大蹼铃蟾蛋白提取物表现出对膀胱癌细胞株有较强的抑制作用 ;对膀胱癌细胞株端粒酶表达则无影响。结论 大蹼铃蟾蛋白提取物对多个膀胱癌细胞株具有直接细胞杀伤作用 ,其作用机制不通过抑制端粒酶活性
Objective To investigate the inhibitory effect of extract of Rhizoma bombularis on bladder cancer cell lines and its effect on telomerase. Methods Tumor cell culture was used to detect the inhibitory effect of extract of RhizomaBoraminopoera on the bladder cancer cell lines T2 4, BIU 87 and ScaBer. The TRAP PCR ELISA was used to detect the effect of extract of Rhizoctonia solani on bladder cancer cell lines Effect of granzyme expression. Results The protein of Rhizoma bombularis showed a strong inhibitory effect on bladder cancer cell lines and had no effect on the expression of telomerase in bladder cancer cell lines. Conclusion The extract of Rhizoctonia solani has a direct cytotoxicity on a number of bladder cancer cell lines, and its mechanism of action is not through inhibition of telomerase activity