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目的研究维生素D受体(vitamin D receptor,VDR)基因TagⅠ和BsmⅠ位点单核苷酸多态性与种植体周围炎易感性的关系。方法采用病例对照试验设计,收集种植体周围炎组及无种植体周围炎组各50例,应用聚合酶链反应—限制性内切酶片段长度多态性分析方法研究2组VDRTagⅠ和BsmⅠ位点的基因型以及等位基因分布特点,并探讨其与种植体周围炎的相关性。结果 VDR基因BsmⅠ位点各基因型(χ2=3.184,P=0.074)和等位基因频率(χ2=3.269,P=0.071)在2组间分布差异无统计学意义。VDR基因TagⅠ位点主要基因型为TT纯合子和Tt杂合子,2种基因型(χ2=4.337,P=0.037)和T、t等位基因频率(χ2=3.907,P=0.048)在2组的分布差异具有统计学意义。TT型在种植体周围炎组中较为多见。结论避免吸烟、牙周炎病史等混杂因素后,种植体周围炎与VDRTaqI位点单核苷酸多态性间存在遗传相关性,尚不能认为与BsmⅠ位点的多态性相关。
Objective To investigate the relationship between single nucleotide polymorphism (SNP) TagI and BsmI of vitamin D receptor (VDR) gene and susceptibility to peri-implantitis. Methods A case-control study was designed. The peri-implantitis group and the peri-implantitis group were collected. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the VDRTagⅠand BsmⅠ Genotype and allele distribution characteristics, and explore the relationship with peri-implantitis. Results There was no significant difference in the distribution of BsmⅠ genotype (χ2 = 3.184, P = 0.074) and allele frequency (χ2 = 3.269, P = 0.071) between the two groups. The major genotypes of TagⅠ in VDR gene were TT homozygote and Tt heterozygote. The frequencies of two genotypes (χ2 = 4.337, P = 0.037) and T and t allele (χ2 = 3.907, P = 0.048) The distribution differences were statistically significant. TT type in the peri-implantitis group more common. Conclusion There is a genetic correlation between peri-implantitis and single nucleotide polymorphism at VDRTaqI locus after avoiding confounding factors such as smoking and history of periodontitis. However, it can not be considered to be associated with polymorphism at BsmⅠ locus.