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目的 了解细胞接受辐射后 ,线粒体DNA(mtDNA)对细胞放射敏感性的影响。方法 利用缺乏mtDNA的细胞株 (ρ0 细胞 )、携带变异mtDNA的细胞株 (syn 细胞 )和携带正常mtDNA的细胞株 (ρ+细胞 )进行体外实验研究。 3种细胞株经不同剂量伽玛射线照射后 ,采用集落形成实验和流式细胞术等方法 ,获得 3种细胞株的存活曲线 (应用LQ模型 ) ,并分析其细胞周期的变化和比较各种细胞之间凋亡的差异。结果 3种细胞株的存活曲线表明 ,ρ0 细胞株比其它 2种细胞株显示对射线有较强的抵抗 (F =10 .45 ,P =0 .0 2 2 )。 2、5和 8Gy照射之后 ,ρ0 细胞株的细胞凋亡明显少于其它 2种细胞株 (F =14.2 9,P =0 .0 0 0 7;F =4.80 ,P =0 .0 2 93;F =4.98,P =0 .0 15 4)。 3种细胞株被照射后细胞周期的变化不明显。结论 mtDNA可能是影响细胞放射敏感性的一个重要因素
Objective To understand the effect of mitochondrial DNA (mtDNA) on the radiosensitivity of cells after irradiation. Methods In vitro studies were performed using cell lines lacking mtDNA (p0 cells), cell lines carrying variant mtDNA (syn cells), and cell lines carrying normal mtDNA (p+ cells). After the three cell lines were irradiated with different doses of gamma radiation, colony formation assays and flow cytometry were used to obtain the survival curves of three cell lines (using the LQ model), and their cell cycle changes and comparisons were analyzed. Differences in apoptosis between cells. Results The survival curves of the three cell lines showed that the ρ0 cell line showed stronger resistance to radiation than the other two cell lines (F = 10.45, P = 0.022). After 2, 5, and 8 Gy irradiation, the apoptosis of ρ 0 cell line was significantly less than that of the other two cell lines (F =14.2 9, P =0. 0 0 0 7; F = 4.80, P =0. 0 2 93; F = 4.98, P =0. 0 15 4). The cell cycle changes were not obvious after the three cell lines were irradiated. Conclusion mtDNA may be an important factor affecting cell radiosensitivity