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A simple,rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis(C.sinensis)a natural plant with anti-hyperglycemic effect,was developed and validated by reversed phase liquid chromatography.Chromatographic separation was achieved on a C18 column and an isocratic elution was carried out at a flow-rate of 1.0 mL·min~(-1) with the acetonitrile-water containing0.1%formic acid(19∶81,v/v).The detection wavelength was set at 330 nm.The calibration curve was linear over the concentration range of 0.25-50μg·mL~(-1) with correlation coefficients larger than0.999 5.The limit of detection and limit of quantification were 0.09μg·mL~(-1)and 0.25μg·mL~(-1),respectively.The precisions and accuracy for all samples were acceptable.The validated method has been successfully applied for the quantification of camellianin B in C.sinensis harvested in different months and may also be used as the quality evaluation of this herb medicine.
A simple, rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis was developed and validated by a reversed phase liquid chromatography. Chromatographic separation was achieved on a C18 column and an isocratic elution was carried out at a flow-rate of 1.0 mL · min ~ (-1) with the acetonitrile-water containing 0.1% formic acid (19:81, v / v) The calibration curve was linear over the concentration range of 0.25-50 μg · mL -1 with correlation coefficients larger than 0.99 5. The limit of detection and limit of quantification were 0.09 μg · mL -1 and 0.25 μg · ML ~ (-1), respectively. These precisions and accuracy for all samples were acceptable. The validated method has been applied applied for the quantification of camellianin B in C.sinensis harvested in different months and may also be used as the quality evaluation. of this herb medicine.