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已知卵巢有多种来源的血纤维蛋白溶酶原激活物(PA)、作者等在1985年巳发现卵巢颗粒细胞和卵泡膜细胞产生不同类型的PA。刺激颗粒细胞主要产生组织型PA(t-PA),而卵泡膜细胞主要分泌尿激酶型PA(u—PA)。颗粒细胞的制备和培养按Crisp和Denys(1975年)的修改法,对大鼠注射孕马血清促性腺激素(PMSG)后,经48小时杀死。分离收集卵泡或卵丘—卵母细胞复合物,DME配方用新配的培养基液洗涤,卵细胞的细胞培养密度为0.7×10~5/细胞/每孔—7mm,加促性腺激素F—SH,LH处理培养细胞后,经24小时,在加入激素的不同时间,测收集液PA的酶活性。
Known ovaries have multiple sources of plasminogen activator (PA), the authors in 1985 have found that ovarian granulosa cells and theca cells produce different types of PA. Stimulation of granulosa cells primarily produces tissue-type PA (t-PA), whereas theca cells secrete urokinase-type PA (u-PA). Preparation and cultivation of granulosa cells The rats were killed 48 hours after injecting pregnant mare serum human gonadotropin (PMSG), as modified by Crisp and Denys (1975). The follicles or cumulus-oocyte complexes were separated and collected. The DME formula was washed with a freshly prepared medium, and the cell culture density of the egg cells was 0.7 × 10 5 / cell / per well-7 mm. The gonadotropin F-SH After LH treatment of the cultured cells, the enzyme activity of the collected liquid PA was measured at different times of addition of hormones over a period of 24 hours.