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将宁杞１号枸杞叶片纵向切开，再横向断成小块，接种在ＭＳ＋６－ＢＡ１ｍｇＬ－１＋ＩＢＡ０．５ｍｇＬ－１的培养基上诱导愈伤组织。将诱导出的愈伤组织分割成０．５ｃｍ×０．５ｃｍ的小块，用秋水仙素加２％的二甲基亚砜混合液进行处理，后转接在ＭＳ＋６－ＢＡ０．５ｍｇＬ－１＋ＩＢＡ１ｍｇＬ－１培养基上培养，２５～３０ｄ即可诱导出小苗。对诱变苗进行细胞学观察，发现染色体变异株系及时转接扩繁，镜检与多倍体无性系的建立同步进行。研究证明，利用愈伤组织进行枸杞同源四倍体的诱导方法简便可靠、诱变频率高、效果好，是创造枸杞新种质、培育枸杞新品种的有效途径。
The Ningqi No.1 wolfberry leaves were longitudinally cut, then cut into small pieces transversely and inoculated on the medium of MS + 6-BA1mgL-1 + IBA0.5mgL-1 to induce the callus. The induced callus was divided into small pieces of 0.5cm × 0.5cm, treated with colchicine plus 2% DMSO, and then transferred into MS + 6-BA0.5mgL-1 + IBA1mgL- 1 medium, 25 ~ 30d can be induced to shoots. Cytological observation of the mutagenic plantlets showed that the chromosomal variation lines were timely transferred and expanded, and the microscopic examination and the establishment of the polyploid clones were carried out synchronously. Studies have shown that using callus of Lycium tetraploid induction method is simple and reliable, high mutagenesis, the effect is good, is to create a new germplasm of Lycium barbarum, an effective way to cultivate new varieties of Lycium barbarum.