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本文通过f_2与指标菌、T_2噬菌体及Polio 1型和Echo 1型病毒对氯、“69-1”和有机碘消毒剂的耐力比较,表明f_2噬菌体对常用卤素消毒剂比指标菌及T_2噬菌体耐力要强,与肠道病毒相近,可以用作水中肠道病毒灭活初步评价的指标。采用JU型玻璃纤维滤纸吸附-洗脱法定量检验水中f_2噬菌体,通过加CaCl_2及调节水样pH至3.0~3.5,促进吸附作用,并以pH8~9.5%牛肉膏汤过滤洗脱,可浓集水样1L,对人工污染水平均回收率为34~102%,总平均58%。
In this paper, the comparison of endurance of f_2 with index bacteria, T_2 bacteriophage, Polio type 1 and Echo 1 virus, chlorine, “69-1” and organic iodine disinfectant showed that bacteriophages f_2 performed better than common halogens disinfectants than indicator bacteria and T_2 bacteriophage Stronger, and similar to enterovirus, can be used as indicators of initial evaluation of waterborne enterovirus inactivation. Adopting JU glass fiber filter paper to adsorb and elute the f_2 bacteriophages in water, quantitatively detecting the bacteriophage f_2 in water by adding CaCl 2 and adjusting the pH value of the water sample to 3.0-3.5, promoting the adsorption and washing with pH8-9.5% Water samples 1L, the level of artificial pollution were 34 ~ 102% recovery, with a total average of 58%.