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目的：观察胃癌细胞内原癌基因ｅｒｂＢ２表达降低前后信号转导与转录激活分子（ＳＴＡＴ１）和细胞周期蛋白Ｄ１（ｃｙｃｌｉｎＤ１）对表皮生长因子（ＥＧＦ）刺激的反应性。方法：采用Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ检测ｅｒｂＢ２表达下调前后及ＥＧＦ刺激前后ＳＴＡＴ１和ｃｙｃｌｉｎＤ１水平。结果：ｅｒｂＢ２表达抑制对ＳＴＡＴ１的ＥＧＦ反应性无显著影响；ｅｒｂＢ２表达下调后ｃｙｃｌｉｎＤ１的ＥＧＦ可诱导性显著降低。结论：ｅｒｂＢ２表达降低可抑制ＥＧＦ对ｃｙｃｌｉｎＤ１的诱导效应。
Objective: To observe the response of STAT1 and cyclin D1 to epidermal growth factor (EGF) stimulation before and after the decrease of erbB 2 expression in gastric cancer cells. Methods: Western blotting was used to detect the expression of STAT1 and cyclinD1 before and after down-regulation of erbB2 expression and before and after EGF stimulation. RESULTS: The inhibition of erbB2 expression had no significant effect on the EGF reactivity of STAT1; the down-regulation of erbB2 expression significantly decreased EGF induction of cyclinD1. Conclusion: The decrease of erbB2 expression can inhibit the induction effect of EGF on cyclinD1.