白细胞介素１８（Ｉｎｔｅｒｌｅｕｋｉｎ１８，ＩＬ１８）是一种能诱导ＩＦＮγ产生的新型细胞因子，在调节Ｔｈ１型细胞免疫应答中起重要作用。采用逆转录ＰＣＲ从活化的小鼠腹腔巨噬细胞中获得了小鼠ＩＬ１８（ｍＩＬ１８）ｃＤＮＡ，测序鉴定正确的片段经ＥｃｏＲＩ酶切后克隆入ＧＳＴ融合表达载体ｐＧＥＸ２Ｔ，再转化至大肠杆菌ＤＨ５α高效表达，经纯化获得了有活性的ｍＩＬ１８融合蛋白 Interleukin 18 (IL18) is a novel cytokine that induces IFNγ production and plays an important role in the regulation of Th1 cellular immune responses. The mouse IL-18 (mIL-18) cDNA was obtained from activated mouse peritoneal macrophages using reverse transcription PCR. The correct fragment was sequenced and digested with EcoRI and cloned into the GST fusion expression vector pGEX-2T. E. coli DH5α is highly expressed and purified to obtain active mIL-18 fusion protein