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目的:建立测定紫杉醇脂质纳米粒体外释放度的方法,考察紫杉醇脂质纳米粒24h的体外释放度。方法:采用固相萃取-高效液相色谱法。样品经C18固相萃取柱萃取,采用Phenomenex luna C18(250mm×4.6mm,5μm)色谱柱分离,流动相为甲醇-水(80:20),流速为1.0mL.min-1,检测波长为227nm。结果:紫杉醇的检测浓度在2～12μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9993),紫杉醇脂质纳米粒在水杨酸钠(1mol.L-1)-PBS(pH7.4)中24h累积释放度为104.9%,其释放规律符合一级动力方程。结论:所建方法简便、灵敏,干扰小,可用于体外释放的检测。
OBJECTIVE: To establish a method for the determination of in vitro release of paclitaxel liposome nanoparticles and investigate the in vitro release of paclitaxel lipid nanoparticles for 24 hours. Methods: Solid Phase Extraction - High Performance Liquid Chromatography. The sample was separated on a C18 solid phase extraction column and separated on a Phenomenex luna C18 (250 mm × 4.6 mm, 5 μm) column using methanol-water (80:20) at a flow rate of 1.0 mL · min-1 and a detection wavelength of 227 nm . Results: The concentration of paclitaxel showed a good linear relationship with the peak area integral value in the range of 2 ~ 12μg.mL-1 (r = 0.9993). Paclitaxel lipid nanoparticles showed good linearity in sodium salicylate (1mol.L-1) -PBS (pH7.4) 24h cumulative release of 104.9%, its release law in line with the first-order kinetic equation. Conclusion: The established method is simple, sensitive and has little interference and can be used for the detection of in vitro release.