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采用miRCURY基因芯片(v.16.0)分析波动高糖诱导肾小管上皮细胞转分化(epitheli-al-messenchymal transition,EMT)差异表达miRNAs,设定平均上升或下降倍数大于2倍和P<0.05为差异标准。结果发现,基因芯片分析中49个miRNAs表达显著上调,其中6个基因已证实在肾纤维化过程中表达升高;34个miRNAs表达显著下调,其中8个已报道在肾纤维化中表达降低。通过荧光定量PCR(RT-qPCR)验证,结果显示,基因芯片与RT-qPCR两方法分析的结果呈高度相关(r=0.98,P<0.01)。miRNAs差异表达与波动高糖诱导肾小管上皮细胞转分化过程密切相关,且基因芯片与RT-qPCR结果一致,以期为肾纤维化早期诊断与治疗提供新的靶点。
MiRNAs were differentially expressed in EMT induced by fluctuating high glucose using miRCURY gene chip (v.16.0), and the average rise or fall multiple was set more than 2 times and P <0.05 was the difference standard. The results showed that 49 miRNAs were significantly up-regulated in gene chip analysis, of which 6 genes were found to be up-regulated during renal fibrosis; 34 miRNAs were significantly down-regulated, of which 8 were reported to have decreased expression in renal fibrosis. The results of RT-qPCR showed that the results of gene chip and RT-qPCR were highly correlated (r = 0.98, P <0.01). The differential expression of miRNAs is closely related to the process of transdifferentiation of renal tubular epithelial cells induced by high glucose and the gene chip is consistent with the RT-qPCR results in order to provide a new target for the early diagnosis and treatment of renal fibrosis.