论文部分内容阅读
一、组培快繁1、材料花托、茎尖。2、培养条件诱导芽培养基:(1)MS+6-BA2.0+NAA 0.2;(2)1/2MS+6-BA0.1;诱导根培养基:(3)MS+IBA2.0。培养温度25～27℃,每天光照10小时,光照强度约为2000Lx。3、生长与分化初级培养将外植体用自来水和蒸馏水冲洗干净,剪成1.0厘米长的小段,在超净工作台上用0.1%HgCl2水溶液对外植体表面消毒6～8分钟,再用无菌水冲洗3～4次,接种在培养基(1)上。继代培养培养6～7周后,愈伤组织上陆续有小芽分化,此时将带小芽的愈伤组织切成2～4个小块,分别移到新鲜培养基(1)上。培养一段时间后,又有小芽陆续分化。培
First, the rapid propagation of tissue culture 1, material Tori, shoot tip. 2. Incubation medium: (1) MS + 6-BA2.0 + NAA 0.2; (2) 1/2 MS + 6-BA0.1; Induction root medium: (3) MS + IBA2.0. Cultivation temperature 25 ~ 27 ℃, light per day 10 hours, light intensity of about 2000Lx. 3. Growth and Differentiation Primary Culture The explants were rinsed with tap water and distilled water, cut into 1.0 cm long section, the explants surface disinfection with 0.1% HgCl2 aqueous solution on a clean bench for 6 to 8 minutes, then no Wash the bacteria 3 to 4 times and inoculate on medium (1). After 6-7 weeks of subculture and subculture, small calluses were gradually differentiated. At this time, the callus with small buds was cut into 2 to 4 pieces and transferred to fresh medium (1) respectively. After training for a period of time, there are small buds gradually differentiated. Pei