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目的:探讨大承气颗粒药物血清对小鼠肠上皮内淋巴细胞产生/分泌白细胞介素-2(interleukin-2,IL-2)的作用。方法:以不同浓度的大承气颗粒剂灌胃大鼠后不同时间点于无菌条件下采取腹腔静脉血,制备药物血清;分离培养肠上皮内淋巴细胞(intestinal intraepithelial lymphocytes,IELs),以药物血清作用于IELs,设培养液对照、IEL对照、正常血清对照,采用放射免疫分析法测定IL-2。结果:不同浓度大承气颗粒的药物血清在多数时间点的作用明显强于培养液对照、IEL对照及正常大鼠血清对照。正常大鼠血清对照组与IEL对照组的IL-2水平很接近。同时间点的药物血清比较,在30min和12h时,4%大承气颗粒的药物血清作用强;在3h时,40%大承气颗粒的作用最强。4%、20%大承气颗粒的药物血清使小鼠分泌IL-2的作用在1.5h达峰值,在24h出现第二个峰值;40%大承气颗粒药物血清使IEL分泌IL-2的峰值在3h。结论:不同浓度的大承气颗粒制备的药物血清具有使IEL产生/分泌IL-2的作用;该作用涉及到药物本身和药物在体内的活性代谢物和/或其诱导的内源性活性物质的作用。
Objective: To investigate the effects of Dachengqi Granule drug serum on the production/secretion of interleukin-2 (IL-2) in mouse intestinal epithelial lymphocytes. METHODS: Rats were treated with different concentrations of Dachengqi granules and intraperitoneal venous blood was taken under aseptic conditions at different time points to prepare drug serum; Intestinal intraepithelial lymphocytes (IELs) were isolated and cultured for drug use. The serum was applied to IELs, and the culture medium control, IEL control, and normal serum control were set. IL-2 was measured by radioimmunoassay. RESULTS: The effects of Dachengqi Granules with different concentrations of the drug serum at most time points were significantly stronger than those of the culture fluid control, the IEL control, and the normal rat serum control. The level of IL-2 in normal rat serum control group and IEL control group is very close. At the same time, compared with the drug serum, the effect of 4% Dachengqi Granules’ drug serum was strong at 30 minutes and 12 hours, and 40% Dachengqi particles had the strongest effect at 3 hours. 4% and 20% Dachengqi Granules’ drug serum caused the secretion of IL-2 by mice to reach the peak at 1.5 h and the second peak at 24 h; 40% Dachengqi Granule drug serum secreted IL-2 to IEL. The peak is at 3h. CONCLUSIONS: Drug sera prepared at different concentrations of Dachengqi granules have the effect of producing/secreting IL-2 by the IEL; this effect involves the drug itself and the active metabolites of the drug in the body and/or the endogenous active substances it induces. The role.