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目的 探讨CD44V 6在鼻咽癌 (NPC)组织中的表达情况及其与NPC浸润转移的关系。方法 对 2 2 9例NPC存档石蜡病理标本 ,采用免疫组织化学染色技术 ,检测CD44V 6蛋白在正常鼻咽和NPC组织中的表达情况 ,分析CD 44V 6与NPC浸润转移的关系。结果 CD44V 6在正常鼻咽组织中呈阴性表达 ,在NPC组织中为高表达 (总阳性率为 83 .0 % ) ,6 3 .8% (14 6 /2 2 9)为高强度表达。T1N3M0 和TxNxM1组织中CD44V 6阳性率分别是 93 .3 %和 94.4% ,但与其它TNM分期组织比较无显著性差异(P >0 .0 5 )。有颈淋巴结转移与无淋巴结转移、有远处转移与无远处转移及上行型与下行型NPC组织比较 ,其CD 44V 6阳性表达率均无显著性差异 (P >0 .0 5 )。CD 44V 6阳性表达强度与T、N分期级别也无明显关系 (P >0 .0 5 )。结论 CD44V 6蛋白在非癌者鼻咽组织中呈阴性表达 ,在NPC中为高表达。CD44V 6对NPC诊断有辅助意义 ,但CD44V 6表达与NPC周围组织浸润程度、颈部淋巴结转移及临床分期无明显相关性 ,与NPC远处转移的关系和预测意义 ,尚有待进一步研究。
Objective To investigate the expression of CD44V 6 in nasopharyngeal carcinoma (NPC) and its relationship with invasion and metastasis of NPC. Methods The pathological specimens of 219 NPC were archived and the expression of CD44V6 protein in normal nasopharyngeal and NPC tissues was detected by immunohistochemistry. The relationship between CD44V6 and invasion and metastasis of NPC was analyzed. Results CD44V 6 was negatively expressed in normal nasopharyngeal tissues, highly expressed in NPC tissues (total positive rate was 83.0%), and 63.8% (14 6/229) was highly expressed. The positive rates of CD44V 6 in T1N3M0 and TxNxM1 tissues were 93.3% and 94.4%, respectively, but no significant difference compared with other TNM staging tissues (P> 0.05). There was no significant difference in the positive rate of CD44V6 between cervical lymph node metastasis and non-lymph node metastasis, with distant metastasis and without distant metastasis, as well as between NPC and NPC (P> 0.05). The positive expression of CD44V6 also had no significant relationship with T, N stage (P> 0.05). Conclusion CD44V 6 protein is negatively expressed in non-human nasopharyngeal tissues and high in NPC. However, the relationship between the expression of CD44V6 and the degree of infiltrating the surrounding tissues of NPC, cervical lymph node metastasis and clinical stage has no significant correlation with the distant metastasis of NPC, and remains to be further studied.