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本文比较分析了ELSA和ABC两种方法在肿瘤McAb筛选中应用的利弊关系。结果表明,948孔融合杂交瘤细胞用B80和SW1116结肠癌细胞系经ELISA测得阳性孔199个,阳性率21％,其中59孔与人外周血单核细胞和A.B血型红细胞反应100％阴性。这些阳性细胞经ABC组化法染色后仅一孔与肠癌而不与正常大肠切片组织反应。ELISA阴性的749孔细胞,
This paper compared the advantages and disadvantages of the two methods of ELSA and ABC in the screening of tumor McAb. The results showed that 948-well hybridomas were positive for 199 positive clones by B80 and SW1116 colon cancer cell lines by ELISA. The positive rate was 21%, and 59 of these cells were 100% negative with human peripheral blood mononuclear cells and A.B blood group red cells. Only one well of these positive cells was stained with ABC histochemical staining but not with normal colon tissue. 749 negative cells with ELISA,