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目的:研究丹参EN制剂对多器官功能障碍综合征(S-MODS)模型大鼠空肠黏膜上皮细胞凋亡及细胞凋亡调控基因ICEmRNA表达的影响。方法:将55只大鼠随机分为对照组、模型组、能全素组和丹参组。模型组、能全素组和丹参组分别经鼠尾静脉注射内毒素(LPS),建立S-MODS模型。对照组和模型组大鼠除正常喂养外,再用等渗盐水灌胃;能全素组给予EN制剂能全素灌胃;丹参组用丹参EN制剂灌胃。实验期为12 d。采用RT-PCR法检测空肠黏膜上皮细胞的凋亡,观察各组空肠黏膜上皮细胞ICE mRNA基因表达情况。结果:丹参组肠黏膜上皮细胞凋亡数低于模型组,且与能全素组有显著性差异(P<0.05)。丹参组空肠黏膜上皮ICE mRNA表达明显低于模型组(P<0.05),与对照组比较,无显著性差异(P>0.05)。结论:丹参EN制剂可能通过下调ICE mRNA表达,减少肠黏膜上皮细胞的凋亡,进而发挥防治S-MODS的作用。
Objective: To study the effect of salvia miltiorrhiza preparation on the apoptosis of jejunum mucosal epithelial cells and the expression of apoptosis-regulating gene ICE mRNA in rats with multiple organ dysfunction syndrome (S-MODS). Methods: Fifty-five rats were randomly divided into control group, model group, vegan group and Salvia miltiorrhiza group. The model group, the vegan group, and the Salvia miltiorrhiza group were injected with endotoxin (LPS) through the rat tail vein to establish the S-MODS model. Rats in the control and model groups were fed with isotonic saline instead of normal feeding. Rats in the vegan group were given vegan gavage by administration of EN, and Salvia miltiorrhiza. The experimental period is 12 days. The apoptosis of jejunal epithelial cells was detected by RT-PCR. The expression of ICE mRNA in jejunum epithelial cells was observed in each group. Results: The number of apoptotic cells in intestinal mucosa of Danshen group was lower than that of the model group, and there was a significant difference with the vegan group (P<0.05). The expression of ICE mRNA in the jejunal epithelium of Salvia miltiorrhiza was significantly lower than that in the model group (P<0.05). There was no significant difference compared with the control group (P>0.05). Conclusion: Salvia miltiorrhiza EN preparation may reduce the expression of ICE mRNA and reduce the apoptosis of intestinal mucosal epithelial cells, and then play a role in the prevention and treatment of S-MODS.