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分别考察醇提法和水提法对葛根总黄酮提取率的影响,结合对鲜豆油抗氧化性能试验,醇提法提取率是水提法提取率的2倍左右,醇提物比空白样的油脂氧化诱导期延长60%左右,比水提物的氧化诱导期延长8.3%左右,结果醇提法优于水提法。利用正交试验对醇提法的提取条件进行优化,在乙醇浓度30%、料液比1∶10,80℃下回流提取90min时,葛根总黄酮提取率达4.756%。对醇提物进行定性检识和和定性检测。对葛根总黄酮进行抑制邻苯三酚自氧化试验研究。在2.5g/L时对邻苯三酚自氧化抑制率为13.98%。葛根总黄酮抗氧化作用明显,可作为抗氧化剂应用到食品工业中。
The effects of alcohol extraction and water extraction on the extraction rate of total flavonoids from Radix Puerariae were investigated respectively. Combined with the antioxidant activity test of fresh soybean oil, the extraction yield of alcohol extraction was about 2 times higher than that of water extraction. The oxidation-inducing period of oil is prolonged by about 60%, which is about 8.3% longer than the oxidation induction period of water extract. As a result, alcohol extraction is superior to water extraction. The extraction conditions of ethanol extraction were optimized by orthogonal test. The extraction rate of total flavonoids in Pueraria lobata reached 4.756% when the ethanol concentration was 30%, the ratio of solid to liquid was 1:90, and the extract was refluxed for 90 minutes at 80 ℃. Alcohol extract qualitative identification and qualitative detection. Study on Inhibition of Pyrogallol Autoxidation by. At 2.5g / L pyrogallol autoxidation inhibition rate of 13.98%. Pueraria total flavonoids antioxidant significantly, can be used as an antioxidant in the food industry.