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目的探讨Notch1信号通路在人脑胶质瘤中的作用机制。方法应用实时荧光量(real-time)PCR方法检测50例人脑胶质瘤标本和10例正常脑组织中的Notch1及其下游靶基因Hes1以及增殖指标Ki-67及PCNA的mRNA表达。利用Western印迹检测Notch1的活化片段NICD的表达。结果 Notch1 mRNA在人脑胶质瘤和正常脑组织中均可表达,但在人脑胶质瘤中的表达显著高于正常脑组织(P<0.01),随着胶质瘤恶性程度的增高,Notch1及其下游靶基因Hes1的表达也随之升高,两者的变化趋势一致。Ki-67在胶质瘤中的表达升高,与正常脑组织对比差异显著(P<0.01);PCNA在人脑胶质瘤中的表达同样升高,与正常脑组织对比差异显著(P<0.05)。结论 Notch1信号通路可能通过Hes1作用于胶质瘤细胞,促进细胞的增殖,进而参与到肿瘤的形成及恶化。
Objective To investigate the mechanism of Notch1 signaling pathway in human glioma. Methods The mRNA expression of Notch1 and its downstream target gene Hes1, proliferation index Ki-67 and PCNA in 50 human glioma specimens and 10 normal brain tissues were detected by real-time PCR. Western blotting was used to detect the expression of NICD, an activated fragment of Notchl. Results Notch1 mRNA was expressed in human glioma and normal brain tissue, but it was significantly higher in human glioma than in normal brain tissue (P <0.01). With the increase of malignant degree of glioma, Notch1 and its downstream target gene Hes1 expression also increased, the trend of the two changes are consistent. The expression of Ki-67 in glioma was significantly higher than that in normal brain tissue (P <0.01). The expression of PCNA also increased in human glioma, which was significantly different from normal brain tissue (P < 0.05). Conclusion Notch1 signaling pathway may play a role in glioma cells through Hes1, promote cell proliferation, and then participate in tumor formation and deterioration.