我们改进了能用于检测不分裂细胞DNA链断裂与重接的羟磷灰石离心—荧光法。证明了鼠血淋巴细胞能将γ—线引起的DNA断链重接。但受30Gy损伤的细胞修复不完全,继续保温至8小时又发生断裂,同时细胞活力下降,再次肯定我们以前提出的观点:即重接DNA分子的再断裂是一种不可逆的生化变化,它可能是细胞死亡的预兆。 We have improved the hydroxyapatite centrifugation-fluorescence method that can be used to detect DNA strand breaks and rejoins in non-dividing cells. It was demonstrated that mouse blood lymphocytes can reconnect DNA strands caused by gamma rays. However, the repair of cells damaged by 30Gy was incomplete, and continued to incubate until 8 hours later, and the cell viability also decreased, reaffirming our previous point that the rebreak of reattached DNA molecules is an irreversible biochemical change. It is a sign of cell death.