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目的观察不同CO_2气腹压力对裸鼠腹腔恶性肿瘤细胞生长和种植转移的影响。方法术前2 h将5×105个结肠癌HT-29细胞注入裸鼠腹腔内。模拟不同CO_2气腹压力的腹腔镜手术环境,持续1 h。将60只裸鼠随机分为4组,每组15只,分别为0 mmHg组、5 mmHg组、10 mmHg组和15 mmHg组,每组CO_2作用3小时。30 d开腹检查肿瘤种植转移情况,并测量腹腔种植的肿瘤平均质量,了解不同CO_2气腹压力对肿瘤生长和种植转移的影响。结果 4腹腔肿瘤平均质量分别为(566.8±50.4)mg、(616.3±61.2)mg、(777.5±70.5)mg、(960.2±56.7)mg。随气腹压力升高,腹腔内种植的肿瘤质量增加,腹腔内肿瘤种植部位增多,15 mmHg气腹组腹腔内种植的肿瘤质量及肿瘤种植部位均大于其他组(P<0.01)。结论 CO_2气腹压强的增高,会增加结肠癌细胞的侵袭和种植的风险,在临床上应引起重视。
Objective To observe the effects of different pneumoperitoneum pressure on growth and metastasis of malignant cells in peritoneal cavity of nude mice. Methods 5 × 105 colon cancer HT-29 cells were injected intraperitoneally into nude mice 2 hours before operation. Laparoscopic surgery simulating different CO_2 pneumoperitoneum pressure lasted for 1 h. Sixty nude mice were randomly divided into 4 groups with 15 rats in each group: 0 mmHg group, 5 mmHg group, 10 mmHg group and 15 mmHg group. Each group was treated with CO 2 for 3 hours. On the 30th day, tumor growth and metastasis were examined by open laparoscopy. The average tumor mass in peritoneal cavity was measured to understand the effects of different pneumoperitoneum pressure on tumor growth and metastasis. Results The average mass of 4 abdominal tumors were (566.8 ± 50.4) mg, (616.3 ± 61.2) mg, (777.5 ± 70.5) mg and (960.2 ± 56.7) mg, respectively. With the increase of pneumoperitoneum pressure, the mass of tumor grown in peritoneal cavity increased and the site of intra-abdominal tumor implantation increased. The mass of tumor and the site of tumor implantation in abdominal cavity of 15 mmHg pneumoperitoneum group were all larger than those in other groups (P <0.01). Conclusions The increased pneumoperitoneum pressure of CO_2 may increase the invasion and colonization risk of colon cancer cells, which should be paid more attention in clinic.