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目的从视网膜微血管形态学和细胞分子两个水平,观察糖康对糖尿病大鼠视网膜病变的影响并探讨其具体作用机制,为糖康防治糖尿病视网膜病变提供理论依据。方法采用腹腔注射链脲佐菌素(STZ)复制糖尿病模型并随机分为正常组、模型组、糖康高、中和低剂量组和达纳康组,每日灌胃,定期检测大鼠体重和非空腹血糖。第12周末,消化铺片法观察视网膜微血管病理和形态学改变。Western blot检测NF-κB亚基P65在细胞核内的含量。RT-PCR检测其下游靶基因Bax和IL-1β的表达水平。结果与正常组比较,模型组大鼠视网膜呈现较多的无细胞毛细血管条索数(约为正常组的12.16倍)。与模型组比较,各治疗组无细胞毛细血管条索数明显减少(P<0.01)。与正常对照组比较,模型组NF-κB蛋白表达明显上调(P<0.01),各治疗组NF-κBP65蛋白在视网膜组织的表达不同程度降低,以糖康高剂量组最为显著。与正常组比较,模型组大鼠视网膜Bax和IL-1β的mRNA表达明显上调(P<0.01)。与模型组比较,各治疗组大鼠视网膜Bax和IL-1β的mRNA表达均不同程度降低,以糖康高剂量组最为显著。结论糖康能够明显抑制糖尿病大鼠视网膜组织NF-κB活性及其下游靶基因Bax和IL-1β的表达水平,通过多途径、多靶点拮抗视网膜细胞凋亡和炎症反应,保护视网膜组织,延缓了糖尿病视网膜病变的发生、发展。
OBJECTIVE: To observe the effect of Tangkang on retinopathy in diabetic rats and to explore its mechanism of action from the morphological and molecular aspects of retinal microvessels, and provide a theoretical basis for Tangkang’s prevention and treatment of diabetic retinopathy. Methods The model of diabetes was established by intraperitoneal injection of streptozotocin (STZ) and randomly divided into normal group, model group, high glucose, medium and low dose of Dan Kang group, And non-fasting blood glucose. At the end of the twelfth week, the pathological and morphological changes of retinal microvessels were observed by digestion and spreading method. The content of NF-κB subunit P65 in the nucleus was detected by Western blot. RT-PCR detection of its downstream target genes Bax and IL-1β expression levels. Results Compared with the normal group, the retina of the model group showed more number of acellular capillaries (about 12.16 times of the normal group). Compared with the model group, the number of acellular capillaries in each treatment group decreased significantly (P <0.01). Compared with normal control group, the expression of NF-κB protein in model group was significantly increased (P <0.01). The expression of NF-κBp65 protein in retina was decreased to different extent in each treatment group. Compared with normal group, the mRNA expression of Bax and IL-1β in retina of model group was significantly increased (P <0.01). Compared with the model group, the mRNA expression of Bax and IL-1β in retina of rats in each treatment group decreased to different extents, especially in the high-dose sugarconcentration group. CONCLUSION: Tangkang can significantly inhibit the expression of NF-κB and its downstream target genes Bax and IL-1β in the retina of diabetic rats, protect the retina tissue through multi-channel and multi-target antagonistic retinal cell apoptosis and inflammation, delay The occurrence and development of diabetic retinopathy.