论文部分内容阅读
目的通过结扎冠状动脉左前降支建立大鼠急性心肌梗死(acute myocardial infarction,AMI)模型,观察牛磺酸处理对AMI后心室重构的影响。方法将60只8周龄雄性Wistar大鼠随机分为4组,包括假手术组、AMI组、低剂量牛磺酸组和高剂量牛磺酸组,每组各15只。假手术组只将缝线绕过冠状动脉,但不予结扎,其他组大鼠建立AMI模型。低剂量牛磺酸组和高剂量牛磺酸组模型建立后第2天开始每天分别用10 m L/kg生理盐水溶解的300、400 mg/kg牛磺酸灌胃。在模型建立前1 d及模型建立后8周行心脏超声检查,测量左心室收缩末期内径(left ventricular end systolic diameter,LVIDs)、左心室舒张末期内径(left ventricular end diastolic diameter,LVIDd)、左心室后壁舒张末期厚度(left ventricular posterior wall end diastolic thickness,LVPWd),计算左心室射血分数(left ventricular ejection fraction,LVEF)、左心室短轴缩短率(fractional shortening,FS),记录二尖瓣口舒张早期最大血流峰值速度(mitral inflow velocity E,E)、舒张晚期最大血流峰值速度(mitral inflow velocity A,A),计算E/A比值,所有检测指标均取6个连续心动周期的平均值。处死动物后测量心脏质量、左心室质量、计算心脏质量指数和左心室质量指数。采用酶联免疫吸附剂测定法测定模型建立前及模型建立后8周血浆脑钠肽水平。结果使用牛磺酸的两组与AMI组比较,心脏质量指数、左心室质量指数、LVIDd、LVIDs显著降低,而LVPWd、LVEF、FS、E/A显著增高(P<0.05),其中以高剂量牛磺酸组更明显(P<0.05)。模型建立成功后AMI组及牛磺酸处理的两组血浆中脑钠肽水平较假手术组高(P<0.05),以AMI组增高更为明显,高剂量牛磺酸组比低剂量牛磺酸组低,差异有统计学意义(P<0.05)。结论牛磺酸干预能减轻大鼠AMI后心室重构,改善左心室功能,该作用与牛磺酸的剂量有关。
Objective To establish an acute myocardial infarction (AMI) model by ligating left anterior descending coronary artery in rats and observe the effect of taurine on ventricular remodeling after AMI. Methods Sixty male Wistar rats of 8 weeks old were randomly divided into 4 groups, including sham operation group, AMI group, low dose taurine group and high dose taurine group, 15 rats in each group. In the sham operation group, only the suture was passed around the coronary artery but not ligated. The AMI model was established in the other groups. The low dose taurine group and the high dose taurine group were orally administered with 300,400 mg / kg taurine dissolved daily with 10 m L / kg normal saline on the second day after establishment. Left ventricular end systolic diameter (LVIDs), left ventricular end diastolic diameter (LVIDd), left ventricular diameter (LVIDd), left ventricular end diastolic diameter (LVIDd) were measured at 1 d before model establishment and 8 weeks after model establishment. Left ventricular posterior wall thickness (left ventricular posterior wall end diastolic thickness, LVPWd), left ventricular ejection fraction (LVEF), left ventricular fractional shortening (FS) The mitral inflow velocity E (E), mitral inflow velocity A (A), and the E / A ratio were calculated. All the detection parameters were averaged over 6 consecutive cardiac cycles value. After the animals were sacrificed, cardiac mass, left ventricular mass, and heart mass index and left ventricular mass index were calculated. Enzyme-linked immunosorbent assay was used to determine the levels of plasma brain natriuretic peptide before and after 8 weeks of model establishment. Results Compared with AMI group, the indexes of cardiac mass index, LVMI, LVIDs, LVPWd, LVEF, FS and E / A were significantly increased in both groups (P <0.05) Taurine group was more obvious (P <0.05). After successful establishment of the model, the levels of plasma brain natriuretic peptide in AMI group and taurine-treated group were significantly higher than those in sham-operated group (P <0.05), and increased more significantly in AMI group than in low-dose taurine Acid group was low, the difference was statistically significant (P <0.05). Conclusion Taurine can attenuate ventricular remodeling and improve left ventricular function after AMI in rats, which is related to the dosage of taurine.