小鼠皮肤在化学致癌物的启始和促进作用下可导致皮肤良性乳头状瘤,其中只有非常少的比例可转变成癌。目前研究这种恶性转变的内在遗传变化只限于体内。本文作者意图建立含有病毒瘤基因特定突变的表皮细胞系,以这种细胞系用于研究促进肿瘤作用的生物学机理,分析良性肿瘤转变为恶性肿瘤的机关遗传变化。 The skin of a mouse can cause benign papilloma of the skin under the initiation and promotion of chemical carcinogens, of which only a very small proportion can turn into cancer. The current intrinsic genetic alterations that underlie this malignant transformation are limited to the body. The authors intended to establish an epidermal cell line containing a specific mutation in the viral tumor gene. This cell line was used to study the biological mechanism that promotes tumorigenesis and to analyze the genetic changes in benign tumors that turn into malignant organs.