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癌的光化学疗法是把肿瘤亲合性高的光敏性物质聚集于肿瘤,通过光照时有选择性地使肿瘤坏死。作者在治疗肝肿瘤中从肝动脉注射碘油,使其有选择性地聚集于肝肿瘤,以碘油为载体,实验研究了使光易感性物质聚于肿瘤的可能性。材料与方法:在体重约200g的SD系鼠皮下与体重约3kg的NZW兔肝脏分别移植MRMT肿瘤(鼠乳癌)和VX-2肿瘤,制作固型肿瘤备用。光易感性物质用脱镁叶绿酸a(PPA)。经静脉投给鼠水溶性PPA4mg/kg。NZW-免在戊巴比妥钠麻醉下,从胃十二指肠向固有肝动脉注射溶解于碘油的PPA。在给药6、24小时后,用下述方法检测肝、肾、脾、肺、肿瘤及血浆中的PPA。处死鼠及NZW兔后,把各脏器匀浆用甲醇提取,经分光光度计测定吸光度,依据标准曲线定量求得PPA。测定值用均值±SE表
The photochemotherapy of cancer is to gather tumor-affecting, light-sensitive substances in the tumor and selectively necros the tumor when exposed to light. In the treatment of liver tumors, the authors injected lipiodol from the hepatic artery to selectively accumulate lipopolysaccharide. Using lipiodol as a carrier, the authors studied the possibility of sensitizing light to the tumor. Materials and Methods: MRMT tumors (rat breast cancer) and VX-2 tumors were transplanted into NZW rabbit livers with a body weight of about 200 g and SD rats with a body weight of about 200 g, and solid tumors were prepared. Light susceptibility material is pheophorbide a (PPA). Rats were intravenously administered with water-soluble PPA 4 mg/kg. NZW-Prevention of PPA dissolved in lipiodol from the gastroduodenal to the intrinsic hepatic artery under pentobarbital sodium anesthesia. After 6, 24 hours of administration, PPA in liver, kidney, spleen, lung, tumor, and plasma was detected by the following method. After the rats and NZW rabbits were sacrificed, the homogenates of each organ were extracted with methanol, the absorbance was measured by a spectrophotometer, and the PPA was quantified on the basis of a standard curve. Measured value with mean±SE table