基于头孢氨苄 (CEX)降解产物中有还原性的巯基 (SH)基团 ,在 0 .2 mol/ L 的 H2 SO4介质中 ,能与Ce( )产生化学发光反应 ,罗丹明 6 G可敏化其发光强度 ,结合流动注射技术 ,考察了本体系的化学发光条件 ,建立了一种测定头孢氨苄的化学发光新方法。该法线性范围为 0 .0 5— 10 .0 μg/ m L,相关系数 r=0 .9984 ,检出限为 0 .0 2 μg/ m L,对 1.0 μg/ m L的头孢氨苄标准溶液进行连续 9次平行测定 ,相对标准偏差为 1.4 %。 Based on the reductive thiol (SH) groups in the degradation products of cefalexin (CEX), chemiluminescence reaction with Ce () in H2SO4 medium of 0.2 mol / L, sensitization of rhodamine 6 G Its luminescence intensity, combined with flow injection technique, investigated the chemiluminescence conditions of this system and established a new chemiluminescence method for the determination of cephalexin. The linear range of this method was 0.5-10.0 μg / mL, the correlation coefficient was 0.9984, the limit of detection was 0. 02 μg / mL, and 1.0 μg / mL of cefalexin standard solution Nine consecutive parallel assays were performed, with a relative standard deviation of 1.4%.