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目的细胞中端粒（ｔｅｌｏｍｅｒｅ）的长度与细胞寿命的调控密切相关，端粒长度的维持需要端粒酶（ｔｅｌｏｍｅｒａｓｅ）的激活，近年来的研究发现，端粒酶的激活与肿瘤的发生、发展关系密切。本文报道人胃癌和结、直肠癌端粒酶活性。方法采用端粒重复序列扩增法（ｔｅｌｏｍｅｒｉｃｒｅｐｅａｔａｍｐｌｉｆｉｃａｔｉｏｎｐｒｏｔｏｃｏｌ，ＴＲＡＰ）来检测人胃癌（１１例）、结肠癌（９例）和直肠癌（２０例）以及其中３９例相应的癌旁组织中的端粒酶活性。结果在４０例肿瘤组织中，有３６例端粒酶呈阳性（９０％），且与肿瘤发展阶段、恶性程度不相关；而３９例癌旁组织中只有１例阳性。结论端粒酶可能是肿瘤诊断的一个很好的指标，有关端粒酶特性及调控方面研究的深入将有助于发展新的肿瘤确诊方法
The length of telomere in the target cell is closely related to the regulation of cell life. The maintenance of telomere length requires the activation of telomerase. Recent studies have found that the activation of telomerase and the occurrence and development of tumors. Close relationship. This paper reports human telomerase activity in gastric cancer and colorectal cancer. Methods Telomeric repeat amplification protocol (TRAP) was used to detect telomerase in human gastric cancer (11 cases), colon cancer (9 cases) and rectal cancer (20 cases), and 39 of them were corresponding adjacent tissues. active. Results Among the 40 tumor tissues, 36 cases were positive for telomerase (90%), and were not related to the stage of development of the tumor and the degree of malignancy. Only 39 cases of cancerous tissue were positive. Conclusions Telomerase may be a good indicator for tumor diagnosis. The in-depth study on the characteristics and regulation of telomerase will contribute to the development of a new method for the diagnosis of tumors.