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蝴蝶兰幼叶离体培养直接诱导体细胞胚胎的发生并进一步发育成原球茎和分化成苗。体细胞胚胎发生起源于上表皮细胞或上表皮下方的叶肉细胞,为单细胞起源。单细胞原胚分裂形成多细胞原胚,历经球形胚、梨形胚、心形胚和子叶胚的发育过程,最终成为较大颗粒状的原球茎。较高浓度的苄基腺嘌呤(6-BA)和腺嘌呤硫酸盐(AdSO4)配合使用能有效诱导体细胞胚胎的发生,最高诱导率可达40%。适当降低6-BA和AdSO4浓度有利于原球茎分化成苗,但两者浓度过低苗的生长发育会受到影响。
In vitro culture of Phalaenopsis leaves direct induction of somatic embryogenesis and further development of the protocorm and differentiation into seedlings. Somatic embryogenesis originates from the mesothelial cells or mesophyll cells beneath the epidermis, originating from a single cell. Single-cell protoplasts divide to form multicellular proembryos, culminating in the development of globular embryos, pear-shaped embryos, heart-shaped embryos and cotyledonary embryos, eventually becoming larger, granular protocorms. The higher concentration of benzyl adenine (6-BA) and adenine sulfate (AdSO4) can effectively induce somatic embryogenesis, the highest induction rate of up to 40%. Appropriate reduction of 6-BA and AdSO4 concentration is conducive to the differentiation of protocorms into seedlings, but the concentration of both too low seedling growth and development will be affected.