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盆栽试验以黄瓜为主栽作物,分蘖洋葱为套作作物,研究了套作不同化感潜力分蘖洋葱对黄瓜生长及土壤微环境的影响.结果表明:与化感潜力强的分蘖洋葱套作,黄瓜根际土壤电导率降低、pH值提高;根际土壤过氧化氢酶和转化酶活性提高,过氧化物酶活性降低;根际土壤细菌丰富度增加.DGGE条带测序显示,黄瓜根际土壤细菌大多与不可培养的细菌种属具有较高的同源性,共有条带的测序比对推测为放线菌纲和变形菌纲,差异条带的测序比对推测为变形菌纲和酸杆菌纲,红螺旋菌目、酸杆菌属只出现在与化感潜力弱的分蘖洋葱套作的黄瓜根际土壤中.相关分析表明,土壤脲酶与黄瓜幼苗株高、全株干质量、叶面积、DGGE条带数呈显著正相关.综上,与化感潜力强的分蘖洋葱套作,可为黄瓜生长创造良好的根际微环境,显著促进黄瓜幼苗生长.
In potted experiment, cucumber as main crop and tillering onion as intercropping crop were used to study the effects of tillage onions on cucumber growth and soil microenvironment with different allelopathic potentials.The results showed that: Cucumber rhizosphere soil electrical conductivity decreased, pH value increased; Rhizosphere soil catalase and invertase activity increased, peroxidase activity decreased; Rhizosphere soil bacterial abundance increased.DGGE band sequencing showed that cucumber rhizosphere soil Most of the bacteria and non-culturable bacterial species have high homology, sequencing of common bands presumed Actinomycetes and Proteobacteria, sequencing of different bands presumed to be Proteobacteria and Acidobacter In the rhizosphere soil of cucumber with cucumber with low allelopathic potential, the correlation analysis showed that the relationship between soil urease and cucumber seedling height, dry weight of whole plant, leaf area, DGGE bands showed a significant positive correlation.To summed up, with the potential allelopathic tiller onion intercropping, can create good rhizosphere micro-environment for cucumber growth, significantly promote the growth of cucumber seedlings.