1案例资料1.1简要案情2012年12月13日,某镇一村民家被盗,经调查和现场勘验确认遗留在现场的大板瓜子壳为嫌疑人所留,提取该检材要求进行DNA检验。1.2 DNA检验送检瓜子壳多呈两瓣前端张开状态,分析应为进食者以手卡住大板瓜子用门牙所嗑。挑选状态基本一致的瓜子壳数个,剪取尖端,加入5%Chelex-100 120μL及蛋白酶K(20mg/m L)10μL;振荡后56℃孵育1h, 1 Case Information 1.1 Brief Case 2012 年 12 月 13 日, a town of a villagers were stolen from the village, after investigation and on-site inspection confirmed that the left in the scene of large plate melon seed shell left for the suspect, extracting the sample requires DNA testing . 1.2 DNA test Seeds were mostly open two-prong shell open state, the analysis should be eaten by hand to eat large plate melon seeds with incisors. Picked the shell of the same number of melon seeds in a number of state, cut the tip, add 5% Chelex-100 120μL and proteinase K (20mg / m L) 10μL; 56 ℃ incubated for 1h,