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目的对2006—2009年上海市Staphylococcus aureus(S.aureus)食品分离株进行肠毒素基因检测和基因分型研究,以了解肠毒素基因的分布规律及S.aureus的流行特点。方法利用PCR方法检测食品中金黄色葡萄球菌肠毒素基因,包括5种传统肠毒素基因(SEA-SEE)和4种新型肠毒素基因(SEG-SEJ);利用脉冲场凝胶电泳法对49株食品分离株进行基因分型。结果本研究发现49株食品分离株中有19株含有肠毒素基因,16株含有传统肠毒素SEA和SEC,且SEC占93.8%,并检测到新型肠毒素SEG、SEI、SEJ和SEH。PFGE法基因分型显示5株菌不能被分型,其余44株可分为28个基因型,表现为基因型的多样性,且分离自不同时间的菌株具有相同的带型。结论应加强食品中S.aureus的监测分析,为其引起的食物中毒的预防和控制提供科学依据。
Objective To investigate the genotypes and enterotoxigenicity of Staphylococcus aureus (S.aureus) isolated from Shanghai in 2006-2009 to understand the distribution of enterotoxin genes and the prevalence of S. aureus. Methods The Staphylococcus aureus enterotoxin gene in food was detected by PCR, including 5 traditional enterotoxin genes (SEA-SEE) and 4 novel enterotoxin genes (SEG-SEJ). The results of pulse-field gel electrophoresis Food isolates are genotyped. Results In the present study, 19 strains of enterotoxigenic genes were found in 19 isolates, 16 of them contained traditional enterotoxin SEA and SEC, accounting for 93.8% of SEC. New enterotoxins SEG, SEI, SEJ and SEH were detected. Genotyping by PFGE showed that 5 strains could not be typed, and the other 44 strains could be divided into 28 genotypes, showing genotypic diversity, and strains isolated from different times had the same banding pattern. Conclusion The monitoring analysis of S.aureus in food should be strengthened to provide a scientific basis for the prevention and control of food poisoning caused by it.