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本研究通过对几种酶消化后人羊膜基膜(HABM)超微结构改变的观察,发现肝素酶Ⅱ可使HABM表层呈微绒毛状改变,并使基膜致密层和透明层变薄及小空洞等改变。推测肝素酶Ⅱ消化后的HABM,可使表面积相对增加,细胞易于附着。肝素酶Ⅱ的作用,主要是使HABM表面的硫酸乙酰肝素降解,使其内的基板蛋白暴露出神经细胞附着和生长位点,从而能使神经细胞的生长增加150％。而肝素酶Ⅰ、胶原酶Ⅰ和硫酸软骨素酶AC消化后的HABM,失去了支持神经细胞附着和生长的各种因素,结果使神经细胞的生长下降。本文从形态学的角度,支持经肝素酶Ⅱ消化后的HABM是神经细胞粘附和生长的理想支持物。
In this study, we observed the ultrastructural changes of human amniotic membrane basement membrane (HABM) after digestion with several enzymes and found that heparinase II could change the surface of HABM to microvilli and thin the dense and clear layers of the basement membrane Small holes and other changes. It is speculated that heparanase II digested HABM, the surface area can be relatively increased, cells are easy to attach. The role of heparanase II is to degrade heparan sulfate on the surface of HABM, exposing the substrate proteins within the nerve cell attachment and growth sites, thereby increasing the growth of nerve cells by 150%. However, heparinase I, collagenase I and chondroitinase ACA digested HABM lost various factors that support the adhesion and growth of nerve cells, resulting in the decrease of the growth of nerve cells. From a morphological point of view, this article supports HABM digested by heparanase II as an ideal support for neural cell adhesion and growth.