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为了解FasL在诱导EAE淋巴细胞凋亡中发挥的作用 ,我们用髓鞘碱性蛋白 (MBP )致敏小鼠 ,建立动物模型 实验性自身免疫性脑脊髓膜炎 (EAE )。分析了EAE动物淋巴细胞膜上Fas及FasL分子的表达水平 ,检测了分泌的细胞因子 ;用FasL分子诱导了EAE淋巴细胞凋亡。结果表明 ,应用MBP致敏KM小鼠 ,成功地建立了EAE模型 ,KM小鼠的发病率为6 8 3% ,发病程度为 1～ 2分 ;小鼠特异性淋转比对照组高 ,并与发病评分成正比 ;细胞因子分泌水平提示以Th2为主 ;EAE淋巴细胞Fas和FasL的表达及凋亡显著高于对照组 ;FasL诱导淋巴细胞凋亡剂量依赖曲线表明 ,在一个较小的浓度范围内呈现正相关 ,若加入单抗可部分阻断FasL诱导的凋亡。结果提示Fas FasL在EAE动物淋巴细胞凋亡中起了重要作用。
To understand the role of FasL in inducing apoptosis of EAE lymphocytes, we sensitized mice with myelin basic protein (MBP) to establish an animal model of experimental autoimmune meningitis (EAE). The expression of Fas and FasL on the lymphocytes of EAE animal was analyzed and the secreted cytokines were detected. The apoptosis of EAE lymphocytes was induced by FasL. The results showed that the application of MBP-sensitized KM mice successfully established the EAE model, the incidence of KM mice was 68.3%, the incidence of 1 to 2 points; mouse specific lymph node metastasis than the control group and And the incidence score is proportional to; cytokine secretion prompted the Th2-based; EAE lymphocytes Fas and FasL expression and apoptosis was significantly higher than the control group; FasL-induced lymphocyte apoptosis dose-dependent curve showed that at a smaller concentration Within the range of positive correlation, if the addition of monoclonal antibody can partially block FasL-induced apoptosis. The results suggest that Fas FasL plays an important role in the apoptosis of lymphocytes in EAE animal.