目的:测定异亚丙基莽草酸的血浆蛋白结合率。方法:采用超滤法结合高效液相色谱法对异亚丙基莽草酸与犬血浆及人血浆的蛋白结合率进行测定。结果:异亚丙基莽草酸与犬血浆在0.3,0.15 g.L-1,0.5 mg.L-1下的血浆蛋白结合率分别为(4.36±0.02)%,(4.12±0.19)%,(2.23±0.59)%。异亚丙基莽草酸在以上3个浓度下与健康人血浆蛋白结合率分别为(11.23±0.01)%,(10.06±0.69)%,(9.72±0.59)%。结论:异亚丙基莽草酸与血浆蛋白的结合较弱。 Objective: To determine the plasma protein binding rate of isopropylidene shikimic acid. Methods: The protein binding rate of isopropylidene shikimic acid to plasma and human plasma of dogs was determined by ultrafiltration combined with high performance liquid chromatography. Results: The plasma protein binding rates of isopropylidene shikimic acid and canine plasma were (4.36 ± 0.02)%, (4.12 ± 0.19)%, (2.23 ± 0.59)%. Isopropylidene shikimic acid was (11.23 ± 0.01)%, (10.06 ± 0.69)% and (9.72 ± 0.59)% respectively at the above three concentrations. Conclusion: The combination of isopropylidene shikimic acid and plasma protein is weak.