论文部分内容阅读
目的探讨不同剂量1,2二氯乙烷(1,2-DCE)对大鼠肝肾功能的损伤作用。方法选取体重150～180 g雌性Wista r大鼠40只,随机分为对照组(橄榄油)和不同剂量染毒组(50、100、200 mg/kg 1,2-DCE)。大鼠连续灌胃染毒14 d后处死,称量大鼠体重和肝、肾等脏器,计算其脏器系数;检测肝、肾功能及血脂等生化指标;同时观察肝肾组织病理改变。结果 1,2-DCE染毒14 d后,大鼠体重除最高剂量200 mg/kg组降低,差异有统计学意义(P<0.01)外,其他各组均无明显变化。各染毒组大鼠肾的脏器系数较对照组均有不同程度升高(P<0.05);大鼠肝的脏器系数仅在200 mg/kg组较对照组增高(P<0.01)。总胆红素(TBIL)和直接胆红素(DBIL)含量随染毒浓度增加而升高,100 mg/kg组较对照组差异有统计学意义(P<0.05);谷丙转氨酶(ALT)和碱性磷酸酶(ALP)在200 mg/kg组较对照组明显升高(P<0.05),而白蛋白(ALB)和总蛋白(TP)在200 mg/kg组则较对照组明显降低(P<0.01)。高密度脂蛋白(HDL)和胆固醇(CHOL)在染毒后有升高趋势,HDL在50 mg/kg和100 mg/kg组与对照组的差异有统计学意义(P<0.01);CHOL则在100 mg/kg组与对照的差异有统计学意义(P<0.05)。甘油三酯(TG)随染毒浓度先降低后升高,但差异无统计学意义(P>0.05)。肾功能除200mg/kg组尿素氮(BUN)较对照组明显降低(P<0.05)外,其他肾功能指标差异无统计学意义(P>0.05)。病理结果显示高剂量染毒组大鼠肝脏出现肝中央区肝细胞颗粒变性,肾脏出现肾小管水肿及肾小管蛋白管型。结论大鼠灌胃染毒1,2-DCE14 d,可致肝肾脏器损伤。
Objective To investigate the effects of different doses of 1,2-dichloroethane (1,2-DCE) on liver and kidney function in rats. Methods Forty female Wistar rats weighing 150 ~ 180 g were randomly divided into the control group (olive oil) and different dose groups (50, 100, 200 mg / kg 1,2-DCE). The rats were sacrificed after 14 days of continuous intragastric administration and the body weight and the organs such as liver and kidney were weighed and their organ coefficients were calculated. The biochemical indexes such as liver and renal function and blood lipids were detected. The pathological changes of liver and kidney were also observed. Results After the rats were treated with 1,2-DCE for 14 days, the body weight of rats was significantly lower than that of the highest dose of 200 mg / kg group (P <0.01). The organ coefficient of rats in each exposure group increased to some extent compared with the control group (P <0.05). The index of liver organ in rats increased only in 200 mg / kg group compared with the control group (P <0.01). The levels of total bilirubin (TBIL) and direct bilirubin (DBIL) increased with the increase of exposure dose. The difference was statistically significant (P <0.05) between ALT and 100 mg / And alkaline phosphatase (ALP) were significantly higher in the 200 mg / kg group than those in the control group (P <0.05), while albumin (ALB) and total protein (TP) (P <0.01). High density lipoprotein (HDL) and cholesterol (CHOL) tended to increase after exposure. The difference of HDL between 50 mg / kg and 100 mg / kg group and control group was statistically significant (P <0.01) The difference between the 100 mg / kg group and the control group was statistically significant (P <0.05). Triglyceride (TG) first decreased and then increased with the concentration, but the difference was not statistically significant (P> 0.05). In addition to the 200mg / kg group, renal function was significantly lower than that of the control group (P <0.05), while there was no significant difference in other renal function indexes (P> 0.05). The pathological results showed that the liver of rats in the high-dose exposure group had degeneration of hepatocytes, tubular edema and tubular protein in the kidney. Conclusion Gavage of 1,2-DCE for 14 days in rats can cause damage to liver and kidney.