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目的对60Coγ射线诱发人正常肝细胞7702凋亡情况进行研究,为探讨辐射诱导细胞凋亡的机制及辐射诱发基因组不稳定性所产生的延迟效应提供重要的实验依据。方法采用60Coγ射线照射人正常肝细胞7702,利用Annexin-V-PI复染法、流式细胞术对辐射诱导细胞凋亡进行检测。结果首次照射中,照射剂量与细胞凋亡率具有明显的剂量-效应关系;二次照射后的细胞凋亡率与首次照射剂量存在明显的剂量-效应关系;传代细胞的细胞凋亡率与首次照射剂量存在明显的剂量-效应关系。结论电离辐射诱发细胞凋亡存在明显的剂量-效应关系,辐射使细胞敏感性增加,所产生的损伤使整个基因组处于一种不稳定状态,可以传递到细胞的子代中,持续影响子代细胞的遗传效应。
Objective To investigate the apoptosis of 7702 cells induced by 60Coγ ray in human normal liver cells and provide important experimental evidences for exploring the mechanism of radiation-induced apoptosis and the delay effect of radiation-induced genomic instability. Methods Human normal hepatic cell line 7702 was irradiated with 60Coγ ray, and the apoptosis induced by radiation was detected by Annexin-V-PI staining and flow cytometry. Results In the first irradiation, there was a significant dose-effect relationship between irradiation dose and apoptosis rate. There was a dose-response relationship between the apoptosis rate and irradiation dose after secondary irradiation. The apoptosis rate of the passaged cells was similar to that of the first irradiation There was a significant dose-response relationship between irradiation doses. Conclusion Ionizing radiation-induced apoptosis has a dose-effect relationship. Radiation increases the sensitivity of the cells. The damage caused by the ionizing radiation causes the whole genome to be in an unstable state, which can be transmitted to the progenies of the cells and continuously affect the progeny cells The genetic effect.